brazerzkidaisan.blogg.se - Rotor gene 6000 software

ROTOR GENE 6000 SOFTWARE MANUAL
ROTOR GENE 6000 SOFTWARE SOFTWARE

The QIAgility is cost-effective and delivers rapid, high-precision PCR setup, while the QIAsymphony AS is ideal for laboratories performing routine PCR tests on a day-to-day basis.

ROTOR GENE 6000 SOFTWARE MANUAL

You can perform manual reaction setup, or take advantage of QIAGEN’s automated solutions for reaction setup. For all you need to run reactions using Rotor-Discs, choose the Rotor-Disc 100 Starter Kit or the Rotor-Disc 72 Starter Kit. Rotor-Discs can be quickly and easily sealed with plastic film using a Rotor-Disc Heat Sealer. Alternatively, the Rotor-Disc 72 has 72 wells. These extra wells can be conveniently used for more reactions or additional controls. The Rotor-Disc 100 is the equivalent of a 96-well plate with an additional 4 reference wells. Rotor-Discs are circular plates of vertically oriented reaction wells. As well as tubes, Rotor-Discs are available, which offer accelerated setup and higher throughput. Changing the format, by simply switching the snap-fit metal rotor that holds the tubes, takes just seconds. The Rotor-Gene Q supports multiple PCR tube formats to suit a range of needs (see figure “ Flexible formats“).

Small, light, and robust simply place the instrument wherever you like.

No condensation or bubbles in reactions due to rotation.

No optical calibration needed at installation or when the instrument is moved.

Lifetime guarantee on highly stable LEDs, no expensive lamps or lasers to change, no gradual performance loss of light source.

This saves time and costs, and allows you to focus on your research not on keeping the cycler up and running.Ĭonvenient features of the Rotor-Gene Q include: The Rotor-Gene Q is engineered to reduce the need for maintenance and to maximize ease of use.

A specially tuned high-intensity optical HRM channel.

The HRM option for the Rotor-Gene Q includes: The rotary design of the Rotor-Gene Q and its outstanding thermal and optical performance are highly suited to HRM. Previously unknown and even complex sequence variations can be readily detected and characterized in a robust and straightforward way.

PCR products can be discriminated according to sequence, length, GC content, or strand complementarity, down to single base-pair changes. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow

ROTOR GENE 6000 SOFTWARE SOFTWARE

In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). Whether your assay is based on intercalating dyes such as SYBR ® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. Unrivaled optical range enables multiple applications

Fast ramping and negligible equilibration times for short run-times.

Uniform detection eliminating the need for ROX reference dye.

These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways. It also eliminates sample-to-sample variations and edge effects. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure “ Precise real-time PCR analysis“). When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling.

The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure “ Cross-section of the Rotor-Gene Q“). Unique rotary design for outstanding performance